Synopsis
In the abandoned hangar that is the HQ of the ANAD Avengers Edwin Jarvis and Mary Jane Watson have viewed Regent defeat Iron Man and Spider-Man. And the ANAD Avengers are missing and they can't contact any other superteams (it seems Regent may have captured them all). Jarvis can't send IM's other armours in auto-mode because only Tony Stark can do so, as a security measure. Besides which there aren't any handy.
But this makes MJ think of something else. Hunting through the crates that have been sent from Avengers Tower she finds the armoured Iron Spider costume Tony created for Spider-Man before Civil War (the 1st 1). She says she wore an Iron Man armour once (during the Other story arc) and had spider-powers during the Spider Island event, so she figures she can handle it.
Regent is carrying his 2 captives in force bubbles to the Cellar prison that he runs as Augustus Roman. Inside his assistant Dr Stillwell doesn't notice that their latest prisoner Harry Lyman/Osborn hasn't yet been sedated by the liquid rising in his tube. He uses a new app in his Webware wrist-phone to emit a sound pulse which shatters his container.
As he escapes he uses it again to call Spider-Man. This is enough to wake Spidey so that he tries to fight his way out of his bubble. But he's really only distracting Regent so he doesn't sense the attack of the Iron Spider. And the villain loses control of the mentally-created bubbles.
SM joins IS in the attack. Her use of the pet name 'Tiger' clues him in that MJ is in the suit. And all 3 combatants get a feeling of deja vu.
Then the Golden Avenger takes a hand. He and Iron Spider tell Spider-Man to go save Harry. SM hands MJ some fresh web-cartridges because the ones in the IS armour will be well-past their use by date. He leaves the other 2 battling Regent while discussing Roman's desire to save the world from super-battles by locking all super-characters away.
In the Cellar Spidey saves Harry from some guards. Then they find and free the other Spider-Man, Miles Morales. As they break open more tubes Regent loses the relevant transmitted powers. And by the time Miles zaps Stillwell there's a lot of angry people on the march. And Harry looks after his fellow civilian prisoner Betty Brant.
Regent says that he still has enough personal power to deal with Shellhead and Iron Spider. But loads of other heroes come out to play ....
.... and Roman winds up incarcerated in his own jail.
Tony Stark worries about supervillains being freed because Regent used them as a powers source too. SM promises to 'tell' Peter Parker to pay attention to his personal life, and TS promises to treat Mary Jane better than his last assistant Pepper Potts. MJ unmasks and assures Betty and Harry that this was a 1-off appearance.
The next evening Tony, MJ and Jarvis have a private celebration while Peter hosts a party in the Coffee Bean for all his friends and family. But Jay Jameson starts coughing blood and collapses.